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tom20 coding region  (Addgene inc)


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    Addgene inc tom20 coding region
    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
    Tom20 Coding Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tom20 coding region/product/Addgene inc
    Average 93 stars, based on 40 article reviews
    tom20 coding region - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "BRET-Based Mitochondrial Subcompartment Localization Biosensors"

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    Journal: bioRxiv

    doi: 10.1101/2025.10.27.684728

    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
    Figure Legend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

    Techniques Used: Immunofluorescence, Staining

    (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.
    Figure Legend Snippet: (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

    Techniques Used: Immunofluorescence, Staining

    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4
    Figure Legend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

    Techniques Used: Immunofluorescence, Staining



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    tom20 coding region  (Addgene inc)
    93
    Addgene inc tom20 coding region
    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
    Tom20 Coding Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tom20 coding region/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    tom20 coding region - by Bioz Stars, 2026-05
    93/100 stars
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    Image Search Results


    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining

    (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining

    (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

    Journal: bioRxiv

    Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

    doi: 10.1101/2025.10.27.684728

    Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

    Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

    Techniques: Immunofluorescence, Staining